Great Britain Biosynthesis of Thyroglobulin : Incorporation of [ 3 H ] Fucose into Proteins by Rat Thyroids in vitro
نویسنده
چکیده
Previous work (Herscovics, 1969) demonstrated that mannose and galactose are incorporated into the carbohydrate groups of thyroglobulin at different times in the elaboration of this glycoprotein from its subunit precursors. Mannose is incorporated on subunits of thyroglobulin at about the time of their synthesis, whereas galactose is incorporated over 30min later near the time of aggregation of subunits to form non-iodinated thyroglobulin (17-18 S protein). It was concluded that this difference in time is related to the positions of these two sugars in the oligosaccharides of thyroglobulin,
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Radioautographic Study of in Vivo and in Vitro Incorporation of Fucose-3h into Thyroglobulin by Rat Thyroid Follicular Cells
The incorporation of fucose-(3)H in rat thyroid follicles was studied by radioautography in the light and electron microscopes to determine the site of fucose incorporation into the carbohydrate side chains of thyroglobulin, and to follow the migration of thyroglobulin once it had been labeled with fucose-(3)H. Radioautographs were examined quantitatively in vivo at several times after injectio...
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